Background:

Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension (By similarity).

Applications:

ELISA, WB, IHC

Name of antibody:

NGEF

Immunogen:

Fusion protein of human NGEF

Full name:

neuronal guanine nucleotide exchange factor

Synonyms:

EPHEXIN; ARHGEF27

SwissProt:

Q8N5V2

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human tonsil and Human liver cancer

IHC Recommend dilution:

30-150

WB Predicted band size:

82 kDa

WB Positive control:

Rat brain tissue and Mouse brain tissue lysates

WB Recommended dilution:

500-2000