Background:

Phosphatidylcholine (PC) is the most abundant mammalian phospholipid. This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. Another distinct synthetic pathway in nucleated cells converts intracellular choline to phosphatidylcholine by a three-step process. The protein isoforms encoded by this gene localize to the endoplasmic reticulum and mitochondria-associated membranes. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms.

Applications:

ELISA, WB, IHC

Name of antibody:

PEMT

Immunogen:

Synthetic peptide of human PEMT

Full name:

phosphatidylethanolamine N-methyltransferase

Synonyms:

PLMT; PNMT; PEAMT; PEMPT; PEMT2

SwissProt:

Q9UBM1

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human colorectal cancer

IHC Recommend dilution:

50-300

WB Predicted band size:

22 kDa

WB Positive control:

Mouse liver tissue, HepG2, 293T and 231 cell lysates

WB Recommended dilution:

1000-5000