Background:

MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 . Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53 . Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 .

Applications:

WB

Name of antibody:

MDM2

Immunogen:

Synthetic peptide of human MDM2

Full name:

MDM2 oncogene, E3 ubiquitin protein ligase

Synonyms：

HDMX; hdm2; ACTFS

SwissProt:

Q00987

WB Predicted band size:

90 kDa

WB Positive control:

293 and Hela cells

WB Recommended dilution:

500-1000